Molecular cloning of DNA complementary to rat alpha 2-macroglobulin mRNA.
نویسندگان
چکیده
منابع مشابه
Molecular cloning of complementary DNA to swine pepsinogen mRNA.
Poly(A)-containing RNA was isolated from total RNA of swine gastric mucosa by oligo(dT)-cellulose chromatography, and was translated in a wheat germ cell-free system. Analysis of the translation products by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that pepsinogen was a major translation product and was synthesized as two different molecular forms with apparent molecula...
متن کاملCloning and characterization of DNA complementary to rat liver UDP-glucuronosyltransferase mRNA.
UDP-glucuronosyltransferase (transferase) clones were isolated from a cDNA bank constructed in pBR322 using transferase-enriched mRNA from the livers of phenobarbital-treated rats. The enrichment of mRNA was accomplished by polysome immunoadsorption with antibody to purified mouse liver transferase. This antibody was shown to bind specifically to rat transferase by Ouchterlony double diffusion ...
متن کاملSynthesis, molecular cloning, and restriction analysis of DNA complementary to vitamin D-dependent calcium-binding protein mRNA from rat duodenum.
The mRNA coding for rat intestinal calcium-binding protein, a vitamin D3-induced protein (Mr 7500), has been partially purified from growing rat duodenum. Double-stranded DNA synthesized from the purified mRNA preparation was inserted into the PstI site of pBR322, using the oligo(dG-dC) tailing procedure. Clones containing DNA complementary to vitamin D-dependent calcium-binding protein mRNA we...
متن کاملMolecular cloning and sequencing of DNA complementary to chicken liver fatty acid synthase mRNA.
The cDNA corresponding to 4.18 kilobases (kb) of the mRNA of chicken liver fatty acid synthase has been cloned and sequenced. The cDNA corresponds to the 3' end of the mRNA and consists of a 1.87-kb noncoding tail and a 2.31-kb region encoding 769 amino acids of the C terminus of the enzyme. The thioesterase at the C terminus, preceded by the acyl carrier protein, can be identified from known a...
متن کاملCloning of DNA Complementary to Rat Liver NADPH-Cytochrome
NADPH-cytochrome c oxidoreductase and cytochrome P-450b mRNAs were enriched from tightly membrane-bound polysomes isolated from the livers of rats administered phenobarbital. This was accomplished by polysome immunoadsorption with monospecific I g G directed toward the oxidoreductase and cytochrome P-450b in conjunction with Staphylococcus aureus ghosts and oligo(dT)-cellulose chromatography. D...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1985
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)38706-9